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Image Search Results
Journal: Scientific Reports
Article Title: Impaired Function of Peripherally Induced Regulatory T Cells in Hosts at High Risk of Graft Rejection
doi: 10.1038/srep39924
Figure Lengend Snippet: Corneal grafts were transplanted onto inflamed high-risk or control (‘low-risk’) host beds, and their corneas and draining lymph nodes (dLNs) were analyzed 14 days post-transplantation. ( A , B ) Corneal grafts were harvested and digested with collagenase D. Single cell suspensions were stained for Foxp3 and CD4, and analyzed using flow cytometry. 5 corneas per group were pooled for each analysis; data shown are representative of 3 independent experiments. ( C) Corneal grafts were harvested, mRNA was isolated, and Foxp3 expression was analyzed using real-time PCR 14 days post-transplantation. n = 5. ( D ) DLNs were harvested, each DLN was stained for Foxp3 and CD4, and analyzed separately using flow cytometry. Mean fluorescence intensity (MFI) of Foxp3 levels by Tregs of low-risk and high-risk graft recipients was assesssed. N = 5 mice/group, data shown are representative of 3 independent experiments. ( E ) Treg suppression assay showing Treg suppressive function of Tregs isolated from DLNs of high-risk vs. low-risk recipients 14 days post-transplantation. Suppression of naïve BALB/c CD4 + CD25 − conventional T cell proliferation by Tregs was assessed following exposure to C57BL/6 (donor) or C3H (third party) APCs. All data were obtained from n = 5 mice/group and representative data from three independent experiments are shown. p values are calculated using the Mann-Whitney test and error bars represent SEM.
Article Snippet: Real-time PCR was performed using a PCR mix (Taqman Universal PCR Master mix; Invitrogen) and preformulated primers for Foxp3 (
Techniques: Control, Transplantation Assay, Staining, Flow Cytometry, Isolation, Expressing, Real-time Polymerase Chain Reaction, Fluorescence, Suppression Assay, MANN-WHITNEY
Journal: Journal of Internal Medicine
Article Title: Functional and homeostatic defects of regulatory T cells in patients with coronary artery disease
doi: 10.1111/joim.12398
Figure Lengend Snippet: Proportions of total Tregs, nTregs and mTregs based on different gating strategies in whole blood and isolated CD4 + T cells of control subjects and post‐ACS and NSTE‐ACS patients
Article Snippet: Quantitative real‐time PCR amplifications were performed with FAM‐labelled TaqMan primer/probe sets for FOXP3 (
Techniques: Isolation, Control
Journal: Journal of Internal Medicine
Article Title: Functional and homeostatic defects of regulatory T cells in patients with coronary artery disease
doi: 10.1111/joim.12398
Figure Lengend Snippet: Expression of CTLA‐4 and CD39 in nTregs and mTregs from control subjects and post‐ACS and NSTE‐ACS patients
Article Snippet: Quantitative real‐time PCR amplifications were performed with FAM‐labelled TaqMan primer/probe sets for FOXP3 (
Techniques: Expressing, Control
Journal: Journal of Internal Medicine
Article Title: Functional and homeostatic defects of regulatory T cells in patients with coronary artery disease
doi: 10.1111/joim.12398
Figure Lengend Snippet: FOXP3 expression in nTregs and mTregs in control subjects and post‐ACS and NSTE‐ACS patients
Article Snippet: Quantitative real‐time PCR amplifications were performed with FAM‐labelled TaqMan primer/probe sets for FOXP3 (
Techniques: Expressing, Control